电离辐射通过转化生长因子-β-介导的角质层-间质转换来促进癌细胞的侵袭迁移

Int J Radiat Oncol Biol Phys 2011 Dec;81 (5): 1530-7. [IF:4.503]Ionizing radiation promotes migration and invasion of cancer cells through transforming growth factor-Beta-mediated epithelial-mesenchymal transition.Zhou YC , Liu JY , Li J , Zhang J , Xu YQ , Zhang HW , Qiu LB , Ding GR , Su XM , Mei-Shi , Guo GZ .Department of Radiation Oncology, Xijing Hospital Fourth Military Medical University, Xi'an, China; Department of Radiation Medicine, College of Preventive Medicine, Xijing Hospital Fourth Military Medical University, Xi'an, China.第四军医大学西京医院放射科

AbstractTo examine whether ionizing radiation enhances the migratory and invasive abilities of cancer cells through transforming growth factor (TGF-β)-mediated epithelial-mesenchymal transition (EMT). Six cancer cell lines originating from different human organs were irradiated by (60)Co γ-ray at a total dose of 2 Gy, and the changes associated with EMT, including morphology, EMT markers, migration and invasion, were observed by microscope, Western blot, immunofluorescence, scratch assay, and transwell chamber assay, respectively. Then the protein levels of TGF-β in these cancer cells were detected by enzyme-linked immunosorbent assay, and the role of TGF-β signaling pathway in the effect of ionizing radiation on EMT was investigate by using the specific inhibitor SB431542. After irradiation with γ-ray at a total dose of 2 Gy, cancer cells presented the mesenchymal phenotype, and compared with the sham-irradiation group the expression of epithelial markers was decreased and of mesenchymal markers was increased, the migratory and invasive capabilities were strengthened, and the protein levels of TGF-β were enhanced. Furthermore, events associated with EMT induced by IR in A549 could be reversed through inhibition of TGF-β signaling. These results suggest that EMT mediated by TGF-β plays a critical role in IR-induced enhancing of migratory and invasive capabilities in cancer cells.

概要 ：聚焦紫外线否可通过转化内皮细胞-β（TGF-β）-介导的上皮-上皮细胞叠加 (EMT)来促进肿瘤的侵扰迁离。使用总量2Gy(60)Coγ线强光源自全人类器官的6种肿瘤，日志与EMT特别的变化，这之外分别来进行显微镜核心技术，氨基酸印迹方式，免疫荧光核心技术，划痕次测试和Transwell于大次测试来观察并探测细胞组织有机体，EMT标有，侵扰迁离能力等。采用蛋白质六轮免疫吸附法探测这些肿瘤之前TGF-β蛋白高水平，来进行比如说抑制剂SB431542来评估TGF-β频率通路在紫外线EMT之前的主导作用。经过总量为2Gy强光的肿瘤之前存在间叶细胞的理解，与；也强光组相对来说其上皮标有减少，间叶细胞标有增加，同时其侵扰移往能力进一步提高，TGF-β蛋白高水平也降低。进一步发现由A549紫外线诱导的EMT可通过对TGF-β频率抑制时有发生逆转。这些结果表明TGF-β介导的EMT在紫外线诱导进一步提高肿瘤侵扰移往能力之前起着关键主导作用。

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